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DOI: https://doi.org/10.4491/eer.2017.223
A comparative study of three different viability tests for chemically or thermally inactivated Escherichia coli
Seon Yeong Park, and Chang Gyun Kim
Department of Environmental Engineering, INHA University, Incheon 22212, Republic of Korea
Corresponding Author: Chang Gyun Kim ,Tel: +82-32-860-7561 , Fax: +82-32-865-1425, Email: cgk@inha.ac.kr
Received: December 28, 2017;  Accepted: February 24, 2018.
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Three different methods of bacterial viability monitoring were compared to detect chemically or thermally inactivated Escherichia coli. Direct colony enumeration, live/dead bacterial cell staining with a fluorescent dye, and the dehydrogenase activity assay were compared with respect to their ease of use and time required to perform the three different tests. The green (live cell)/red (dead cell) ratio obtained from the fluorescent bacterial cell staining approach showed a linear relationship with the colony forming units; the result obtained with dehydrogenase was similar to those. The sensitivity of the monitoring methods to detect bacterial deactivation varied with different disinfection conditions. After thermal treatment, the sensitivity of the staining approach was lower, while that of the dehydrogenase activity assay was the highest. After chemical treatment, the sensitivity of detection for both methods was similar.
Keywords: Bacterial viability | Confocal laser scanning microscopy | Dehydrogenase | Disinfection | Fluorescence dye staining
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