| KINETICS OF AUTOTROPHIC DENITRIFICATION FOR THE BIOFILM FORMED ON SULFUR PARTICLES : Evaluation of Molecular Technique on Monitoring
Biomass Growth
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| Sungyoun Kim1, Am Jang2, I-Tae Kim1, Kwang-Soo Kim1, and In S. Kim3† |
1Construction Environment Research Department, Korea Institute of Construction Technology, 2311 Daewha-Dong, Usan-Gu, Goyang-Si, Gyeonggi-Do 411-712, Republic of Korea
2Dept. of Civil & Environmental Engineering, The University of Cincinnati, Cincinnati, Ohio, 45221-0071, USA
3Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea |
Corresponding Author:
In S. Kim ,Tel: +82-62-970-2436, Fax: +82-62-970-2434 , Email: iskim@gist.ac.kr
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Received: June 7, 2005; Accepted: December 3, 2005. |
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| ABSTRACT |
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Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, /jmax. and the half velocity constant, KM, were estimated as 0.61 d"1 and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode. |
| Keywords:
Autotrophic denitrification | kinetics | packed-bed | sulfur | biofilm | half-order |
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