| Home | E-Submission | Sitemap | Contact Us
Environ Eng Res > Volume 22(2); 2016 > Article
Wang and Lim: Electric power generation from treatment of food waste leachate using microbial fuel cell

Abstract

Simultaneous treatment of food waste leachate and power generation was investigated in an air-cathode microbial fuel cell. A TCOD removal efficiency of 95.4 ± 0.3% was achieved for an initial COD concentration of 2,860 mg/L. Maximum power density ranged was maximized at 1.86 W/m3, when COD concentration varied between 60 mg/L and 2,860 mg/L. Meanwhile, columbic efficiency was determined between 1.76% and 11.07% for different COD concentrations. Cyclic voltammetric data revealed that the oxidation peak voltage occurred at −0.20 V, shifted to about −0.25 V. Moreover, a reduction peak voltage at −0.45 V appeared when organic matters were exhausted, indicating that reducible matters were produced during the decomposition of organic matters. The results showed that it was feasible to use food waste leachate as a fuel for power generation in a microbial fuel cell, and the treatment efficiency of the wastewater was satisfied.

1. Introduction

During the collection and treatment processes of food waste, large quantity of leachate would produce. In Korea, for example, it was reported as 9,077,000 kg/d in 2007, which was predicted to increase to 12,227,000 kg/d by 2013 [1]. One of treatment strategies for food waste leachate is to discharge it into civil wastewater treatment plants [1]. The strategy can increase the operational cost, and waste valuable energies carried by organic matters and nutrient contents in the food waste leachate.
Tremendous attentions have been paid to microbial fuel cells (MFCs). MFCs could attract electric energy from organic matters, and thus they are promising technology to provide green energy and clean wastewater [2]. Exoelectrogens are with the ability to transfer electrons intracellularly to external electron acceptor, and thus they play core role of transferring electrons attracted from degradation of organic matter to the andic surface to close the process of electric power generation. Because of the extracellular electron transfer process, MFCs could accelerate the removal efficiency of organic matters and even they are able to degrade some persistence organic pollutants [3]. Several kinds of real organic wastewater were successfully treated with MFC technology. Landfill leachate is high strength wastewater. In air-diffusion cathode MFC, 8.5 kg COD m−3d−1 was removed and 344 mWm−3 electric power was generated [4]. Similar treatment efficiency was determined to other kinds of high strength wastewaters, including swine wastewater [5], fermented wastewater [6], brewery wastewater [7, 8], and starch processing wastewater [9]. Food waste leachate contains rich organic matters, such as proteins and alcohol, and thus it should be an alternative substrate for power generation in MFCs. However, the feasibility of food waste leachate as substrate for power generation in MFCs has not yet been reported.
The purpose of the present study, therefore, is to evaluate the possibility of using food waste leachate as substrate for MFCs, and determine the removal efficiency of organic matters. Moreover, the performance of MFCs responding to initial COD concentration was as well compared.

2. Materials and Methods

2.1. MFC Fabrication

The volume of the air-diffusion cathode MFC was 234 mL (6.5 cm × 6 cm × 6 cm), with an effective volume of 200 mL (Fig. 1). The anode was made of carbon felt with a geometrical surface area of 49.6 cm2, and the cathode was a commercially available ELAT® gas diffusion electrode (Lot #LT 120E-W: 090205) with Pt catalyst (20%). Nafion 115 membrane (Alfar, USA) was pretreated as previously described [10]; then heat pressed to the catalyst side of the cathode at 120°C. The membrane side was set to face the anodic solution. Titanium wire was used to connect anode and cathode.

2.2. MFC Inoculation and Operation

The inoculum was collected from the Okchen wastewater treatment plant (Okchen, Korea). To start up the MFC, 20 mL inoculum was mixed with 180 mL of artificial wastewater containing 1.05 g/L NH4Cl, 1.5 g/L KH2PO4, 2.2 g/L K2HPO4, 0.2 g/L yeast extract, and 1.36 g/L CH3COONa·3H2O as electron donors [11]. When the voltage decreased below 30 mV, 0.3 g CH3COONa·3H2O was added to the anodic solution until consecutive stable voltages were achieved at least twice. After the MFC was successfully inoculated, the solution was replaced with food waste leachate. The food waste leachate was kept at 4°C in a refrigerator, and its characteristics were listed in Table 1.
The food waste leachate was diluted to different initial COD concentrations with distilled water, and then buffered with 1.5 g/L KH2PO4 and 2.2 g/L K2HPO4. Cyclic voltammogram (CV) analysis was carried out with three-electrode configuration with anode as working electrode, cathode as counter electrode and Ag/AgCl (3.3 M KCl) as reference electrode. The MFC was performed via batch mode and the anodic solution was stirred with a magnetic stirring bar. During the research, the temperature was controlled at 30 ± 1°C in an incubator.

2.3. Calculations

Both voltage and CV were recorded with a pertentiostat (WMPG 1000, Won-A Tech, Korea). Power density (W/m3) were calculated according to P = V2/R·v, where V is the voltage across the external resistance (R), and v is the effective volume of anode chamber. Current and current density were calculated as I = V/R and J = I/v, respectively. Coulombic efficiency (CE) was calculated as CE = CP/CT, where CP is the practical coulombs calculated by integrating the current over time, and CT is the theoretical coulombs available from removed COD. The internal resistance (Rint) was determined as the slope of I–V curve according to V = E-I·Rint, where E is electromotive force of the cell [12].

3. Results and Discussion

3.1. Voltage Output

With sodium acetate as an electron donor, a stable voltage of about 390 mV across the external resistor was repeatedly obtained (Fig. 2(a), latest 3 cycles). Replacing artificial wastewater with food waste leachate, the voltage rose quickly to stable voltage of 270 mV (Fig. 2(b)). The results demonstrated that organic components of food waste leachate were easily decomposed by exoelectrogens located on the surface of anode. After complete cycles, the TCOD decreased from 2,860 mg/L to 131.9 ± 8.6 mg/L, representing a removal efficiency of 95.4 ± 0.3%. The SCOD decreased by 96.2 ± 0.6% from 2,664 mg/L to 101.2 ± 15.6 mg/L. The CE was used to indicate the recovery efficiency of electrons as electric current from the decomposition of organic matters. It was determined that the CE was 5.79 ± 0.03% in the present study. It was similar to 6.7% to 8% with starch processing wastewater as fuel for MFC [9], however, it was larger than that (< 1%) when fermented wastewater was used for power generation [6]. Low CE indicated that large amount of electrons containing in organic matters was consumed by processes other than power output. Within the electroactive biofilm, many other species of microorganism than exoelectrogens exit, such as methanogenium [6]. The methanogenium could compete with exoelectrogens to take advantage of the organic matters to produce methane instead of electric current, and thus decreasing the CE of MFCs. Moreover, the environment of air-diffusion cathode MFC is not definitely anaerobic, and thus organic matters could be aerobically degraded by microorganism, which could as well reduce the CE [13].
For COD of 2,860 mg/L, a maximum power density was determined as 1.53 ± 0.11 W/m3 at a current density of 8.75 ± 0.31 A/m3 (Fig. 3(a)). It was similar to 1.10 W/m3 for real field dairy wastewater [14], 2.19 W/m3 for animal carcass wastewater [15], and 2.21 W/m3 for sea food processing wastewater as fuel [16], but it was smaller than 8.0 W/m3 for real dye wastewater [17]. Moreover, the Rint was determined as 109.95 ± 3.95 Ω (Fig. 3(b)). Performance of MFCs relies on couples of factors, such as the configuration, electrode material, operation parameter, and characteristics of the real wastewater [18]. The nature of wastewater would affect the community composition of the electroactive biofilm, and further influence the wastewater treatment efficiency and electric power generation [19].

3.2. Effects of COD Concentration

COD concentration could affect the performance of MFCs. Different COD concentrations (60, 110, 275, 580, 878, 2,160, and 2,860 mg/L) were used to evaluate its influence on the electric power generation, Rint, and CE. The solutions were buffered with 1.5 g/L KH2PO4, and 2.2 g/L K2HPO4.
The maximum power density ranged between 1.19 ± 0.04 and 1.86 ± 0.003 W/m3 for different initial COD concentrations (Fig. 4(a)). It was best fitted to the BoxLucas 1 model using OriginPro 7.5 program (R2 = 0.973) according to the equation: P = Pmax(1-e−kCs), where k is the growth rate constant, and Cs is the substrate concentration. It meant that the maximum power density could be easily obtained even at a relatively low concentration of organic matter. At a COD concentration of 580 mg/L, the largest maximum power density (1.86 ± 0.003 W/m3) was obtained due to the smallest Rint of 83.7 ± 0.3 Ω (Fig. 4(b)). Rint was calculated to be between 83.7 ± 0.3 and 116.95 ± 1.55 Ω for different initial COD concentrations.
Based on the same buffer concentration, a high COD concentration (low dilution ratio) led to a high solution conductivity, and an increase in solution conductivity would lower the Rint of MFC [12]. However, such a relation between conductivity and Rint was only observed when the COD concentration was not larger than 580 mg/L. Larger COD concentration than 580 mg/L led to larger Rint. The reason for this might be that the concentration of possibly existing harmful matters was high enough to limit the activity of exoelectrogens with low dilution ratio.
CE could be as well affected by the COD concentration. In the present study, the CE increased from 9.64% for a COD of 60 mg/L to 11.07 ± 1.51% for a COD of 110 mg/L, and then decreased to 1.76 ± 0.06% for a COD of 2,160 mg/L (Table 2, based on 12 h discharge). The inverse relationship between CE and COD concentration was also observed by other research [5]. It was because that higher COD concentration took longer time to be completely decomposed by microorganism, and therefore more oxygen could diffuse to the anodic chamber; more oxygen could provide more electron acceptors for aerobic microorganism to consume the organic matters, which further decreased the CE [5]. Based on the same reaction time in this study, harvested coulomb was not significantly different when the COD was saturated. However, removed COD was positively correlated to initial COD, which led to a low CE for high initial COD. It was implied that other factors besides to oxygen diffusion that could lower CE should be taken into consideration to improve CE when real wastewater was used to generate electric power in MFCs.

3.3. Cyclic Voltammetry

The Cyclic Voltammetry (CV) for initial and treated food waste leachate were performed with an initial COD of 580 mg/L (Fig. 5). Obviously, an oxidation peak was observed for the original food waste leachate at about −0.20 V. After the MFC cycle was complete, the oxidation peak decreased in intensity and shifted to about −0.25 V. The decrease in the oxidation peak was possibly due to the decomposition of organic matters by the microbes, which further led to a substrate limitation to the exoelectrogens. When the organic matter was decomposed, however, the reduction current was increased and a current peak appeared at about −0.45 V. The increased reduction current in the CV curve was likely incurred by the produced reducible matters during the decomposition process of organic matters. Moreover, the peak current voltage of −0.45 V was larger than previously reported voltage of −0.34 V for NAD+/NADH [20]. It is implied that the electroactivity of the biofilm in the present was more robust, probably due to that some exoelectrogens within the biofilm obtained high ability to transfer electrons from the microbe to the anodic surface.

4. Conclusions

In the present research, electric power generation coupled with food waste leachate treatment using air-diffusion cathode MFC was successfully studied. The removal efficiency of COD was determined to be as high as 95.4 ± 0.3%, when the COD concentration was 2,860 mg/L. The maximum power density ranged was maximized at 1.86 W/m3, with COD concentration ranged of 60–2,860 mg/L. CV experiments showed that reducible matters were produced during the process of food waste leachate decomposition and implied that exoelectrogens with high ability to transfer electrons existed within the biofilm on the anode.

References

1. Korean Ministry of Environment. Study on development on optimum models and facilitating/operational guide line according to methods for energy generation of food waste and leachate. Sejong: Ministry of Environment; 2008.


2. Wang Z, Lee T, Lim B, Choi C, Park JMicrobial community structures differentiated in a single-chamber air-cathode microbial fuel cell fueled with rice straw hydrolysate. Biotech Biofuels. 2014;7:9
crossref

3. Wang L, Liu Y, Ma J, Zhao FRapid degradation of sulphame-thoxazole and the further transformation of 3-amino-5-methylisoxazole in a microbial fuel cell. Water Res. 2016;88:322–328.
crossref pmid

4. Puig S, Serra M, Coma M, Cabré M, Balaguer MD, Colprim JMicrobial fuel cell application in landfill leachate treatment. J Hazard Mater. 2011;185:763–767.
crossref pmid

5. Min B, Kim JR, Oh SE, Regan JM, Logan BEElectricity generation from swine wastewater using microbial fuel cells. Water Res. 2005;39:4961–4968.
crossref pmid

6. Nam JY, Kim HW, Lim KH, Shin HSEffects of organic loading rates on the continuous electricity generation from fermented wastewater using a single-chamber microbial fuel cell. Bioresour Technol. 2010;101:33–37.
crossref

7. Wen Q, Wu Y, Cao DX, Zhao LX, Sun QElectricity generation and modeling of microbial fuel cell from continuous beer brewery wastewater. Bioresour Technol. 2009;100:4171–4175.
crossref pmid

8. Wang X, Feng YJ, Lee HElectricity production from beer brewery wastewater using single chamber microbial fuel cell. Water Sci Technol. 2008;57:1117–1121.
crossref pmid

9. Lu N, Zhou S-G, Zhuang L, Zhang J-T, Ni J-RElectricity generation from starch processing wastewater using microbial fuel cell technology. Biochem Eng J. 2009;43:246–251.
crossref

10. Chae KJ, Choi MJ, Ajayi FF, Park WS, Chang IS, Kim ISMass transport through a proton exchange membrane (Nafion) in microbial fuel cells. Energ Fuel. 2008;22:169–176.
crossref

11. Ramasamy RP, Ren ZY, Mench MM, Regan JMImpact of initial biofilm growth on the anode impedance of microbial fuel cells. Biotechnol Bioeng. 2008;101:101–108.
crossref pmid

12. Liu H, Cheng SA, Logan BEPower generation in fed-batch microbial fuel cells as a function of ionic strength, temperature, and reactor configuration. Environ Sci Technol. 2005;39:5488–5493.
crossref pmid

13. Cheng SA, Liu H, Logan BEIncreased performance of single-chamber microbial fuel cells using an improved cathode structure. Electrochem Comm. 2006;8:489–494.
crossref

14. Mohan SV, Mohanakrishna G, Velvizhi G, Babu VL, Sarma PNBio-catalyzed electrochemical treatment of real field dairy wastewater. Biochem Eng J. 2010;51:32–39.
crossref

15. Li X, Zhu N, Wang Y, Li P, Wu P, Wu JAnimal carcass wastewater treatment and bioelectricity generation in up-flow tubular microbial fuel cells: Effects of HRT and non-precious metallic catalyst. Bioresour Technol. 2013;128:454–460.
crossref pmid

16. Jayashree C, Tamilarasan K, Rajkumar M, et alTreatment of seafood processing wastewater using upflow microbial fuel cell for power generation and identification of bacterial community in anodic biofilm. J Environ Manage. 2016;180:351–358.
crossref pmid

17. Kalathil S, Lee J, Cho MHEfficient decolorization of real dye wastewater and bioelectricity generation using a novel single chamber biocathode-microbial fuel cell. Bioresour Technol. 2012;119:22–27.
crossref pmid

18. Rinaldi A, Mecheri B, Garavaglia V, Licoccia S, Di Nardo P, Traversa EEngineering materials and biology to boost performance of microbial fuel cells: A critical review. Energy Environ Sci. 2008;1:417–429.
crossref

19. Chae KJ, Choi MJ, Lee JW, Kim KY, Kim ISEffect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells. Bioresour Technol. 2009;100:3518–3525.
crossref pmid

20. Wang Z, Lim B, Lu H, Fan J, Choi CCathodic reduction of Cu2+ and electric power generation using a microbial fuel cell. Bul Korean Chem Soc. 2010;31:2025–2030.
crossref

Fig. 1
Schematic of the air-cathode MFC reactor.
/upload/thumbnails/eer-22-2-157f1.gif
Fig. 2
Voltage output during the start-up period (a) and food waste leachate as fuel (b) (external resistor of 500 Ω).
/upload/thumbnails/eer-22-2-157f2.gif
Fig. 3
Power densities at different current densities (a) and I–V curve (b) (external resistance from 1,000 Ω to 50 Ω, duplicate data).
/upload/thumbnails/eer-22-2-157f3.gif
Fig. 4
Maximum power density (a) and internal resistance (b) for different COD concentrations (duplicate data).
/upload/thumbnails/eer-22-2-157f4.gif
Fig. 5
CVs for initial and reacted solutions with an initial COD of 580 mg/L (scan rate of 50 mV/s).
/upload/thumbnails/eer-22-2-157f5.gif
Table 1
Characteristics of Food Waste Leachate
Parameters Value
COD, mg/L 29,120 ± 120
pH 4.94 ± 0.01
SS, mg/L 1,920 ± 80
TN, mg/L 742.2 ± 28.2
TP, mg/L 121.8 ± 6.9
NH3-N, mg/L 160.04 ± 2.36
a Conductivity, mS/cm 11.37 ± 0.08
Salinity, ppt 5.5 ± 0.0

a at 32.7 ± 0.3°C

Table 2
Comparison of CE for Different COD Concentration
COD (mg/L) Coulomb (C) Coulombic



Influent Effluent Removed Generated Recovered Efficiency (%)
2,160 1,562.1 ± 3.7 597.9 ± 3.7 1,442.21 ± 8 25.4 ± 0.7 1.76 ± 0.06
878 366.7 ± 17.9 511.0 ± 17.9 1,232.48 ± 43 26.5 ± 0.1 2.15 ± 0.08
580 186.8 ± 0.8 393.3 ± 0.8 948.57 ± 1.8 26.3 ± 0.1 2.77 ± 0.01
275 53.1 ± 5.8 221.9 ± 5.8 535.18 ± 14 26.35 ± 0.15 4.93 ± 0.16
110 34.3 ± 2.1 75.8 ± 2.1 182.72 ± 4.9 20.3 ± 3.3 11.07 ± 1.51
60 30.2 29.8 71.91 6.93 9.64
TOOLS
PDF Links  PDF Links
PubReader  PubReader
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Supplement  Supplement
  E-Mail
Share:      
METRICS
0
Crossref
0
Scopus
1,278
View
28
Download
Editorial Office
464 Cheongpa-ro, #726, Jung-gu, Seoul 04510, Republic of Korea
TEL : +82-2-383-9697   FAX : +82-2-383-9654   E-mail : eer@kosenv.or.kr

Copyright© Korean Society of Environmental Engineers. All rights reserved.                Powerd by M2community
About |  Browse Articles |  Current Issue |  For Authors and Reviewers