| Home | E-Submission | Sitemap | Contact Us |  
Environmental Engineering Research 1998;3(1): 47-53.
Comparison of Microbial Biomass Estimation Techniques for the Initial Rate Kinetic Studies
Bumhan Bae1, Robin L. Autenrieth2, and James S. Bonner2
1Corresponding Author, Department of Civil Engineering, KyungWon University Sungnam, Kyunggi, 461-701
2Department of Civil Engineering, Texas A&M University College Station, TX 77843-3136
Corresponding Author: Bumhan Bae ,
Received: January 20, 1998;  Accepted: February 21, 1998.
Share :  
Five commonly used biomass quantitation techniques were evaluated for a mixed microbial culture grown on phenol as a sole carbon source. These techniques include total suspended solids (TSS), optical density (OD), protein content, cell volume, and adenosine triphosphate (ATP) contents. The results from each technique were compared with consideration that each technique measures different biochemical properties. OD values generated the best correlation to TSS followed by cell volume and protein content. ATP contents showed the poorest correlation with TSS. The cell volume technique and ATP contents were more sensitive than the other techniques tested. Although the cell volume technique required less time for analysis than the ATP technique, the coefficient of variation for measured values was higher than that of other methods tested. However, the cell volume measurement was determined to be the most effective biomass estimation technique for the initial rate experiments based on the time for analysis and the sensitivity compared to the other methods tested. The relative advantages and limitations for the use of these techniques in kinetic studies were discussed.
Keywords: ATP | biomass | cell volume | optical density | protein contents | TSS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Colonization of Microbial Biofilms in Pipeline of Water Reuse  2014 ;19(3)
Editorial Office
464 Cheongpa-ro, #726, Jung-gu, Seoul 04510, Republic of Korea
TEL : +82-2-383-9697   FAX : +82-2-383-9654   E-mail : eer@kosenv.or.kr

Copyright© Korean Society of Environmental Engineers. All rights reserved.        Developed in M2community
About |  Browse Articles |  Current Issue |  For Authors and Reviewers