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Environmental Engineering Research 1998;3(1): 47-53.
Comparison of Microbial Biomass Estimation Techniques for the Initial Rate Kinetic Studies
Bumhan Bae1, Robin L. Autenrieth2, and James S. Bonner2
1Corresponding Author, Department of Civil Engineering, KyungWon University Sungnam, Kyunggi, 461-701
2Department of Civil Engineering, Texas A&M University College Station, TX 77843-3136
Corresponding Author: Bumhan Bae ,
Received: January 20, 1998;  Accepted: February 21, 1998.
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ABSTRACT
Five commonly used biomass quantitation techniques were evaluated for a mixed microbial culture grown on phenol as a sole carbon source. These techniques include total suspended solids (TSS), optical density (OD), protein content, cell volume, and adenosine triphosphate (ATP) contents. The results from each technique were compared with consideration that each technique measures different biochemical properties. OD values generated the best correlation to TSS followed by cell volume and protein content. ATP contents showed the poorest correlation with TSS. The cell volume technique and ATP contents were more sensitive than the other techniques tested. Although the cell volume technique required less time for analysis than the ATP technique, the coefficient of variation for measured values was higher than that of other methods tested. However, the cell volume measurement was determined to be the most effective biomass estimation technique for the initial rate experiments based on the time for analysis and the sensitivity compared to the other methods tested. The relative advantages and limitations for the use of these techniques in kinetic studies were discussed.
Keywords: ATP | biomass | cell volume | optical density | protein contents | TSS
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