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Environmental Engineering Research 2000;5(3): 175-181.
HYDROGEN PRODUCTION FROM SUCROSE BY ANAEROBIC MICROFLORA
Young Joon Lee
Water Quality Research Department, National Institute of Environmental Research, Inchon 404-170, Korea
Corresponding Author: Young Joon Lee ,
Received: July 14, 2000;  Accepted: September 15, 2000.
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ABSTRACT
Batch fermentations were performed with mesophilic mixed microorganisms. The initial sucrose concentrations were controlled to evaluate its influence on hydrogen production quantitatively. Nine hydrogen production curves were generated, and individual curve was fitted using nonlinear regression analysis with the Gompertz equation. This function was a suitable model for describing the progress of cumulative hydrogen production. There was no significant methane found in the biogas. The hydrogen production yield was 29.0 mL/g sucrose when the initial sucrose concentration was 5 g/L. However, it dropped to 2.5 mt./g sucrose when the initial sucrose concentration exceeded 100 g/L. The production yields of VFAs and solvents were similar to the results of hydrogen. The influence of high initial sucrose concentration on individual specific hydrogen production rate was analyzed using the modified Haldane equation. Through the analysis of the Haldane equation, maximum specific hydrogen production rate, half-saturation constant and inhibition constant approximated 3.933 mL'g VSS/h, 7.457 g sucrose'L and 49.518 g sucrose/L, respectively.
Keywords: anaerobic treatment | hydrogen production | inhibition | sucrose
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