| Home | E-Submission | Sitemap | Contact Us |  
Environmental Engineering Research 2007;12(4): 136-147. DOI: https://doi.org/10.4491/eer.2007.12.4.136
EFFECTS OF THE HERBICIDE, BUTACHLOR, ON NITROGEN FIXATION IN PHOTOTROPHIC NONSULFUR BACTERIA
Kyung Mi Lee1, Jaisoo Kim2, and Hyun Soon Lee1
1Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea
2Department of Life Science, Kyonggi University, Suwon 443-760, Korea
Corresponding Author: Hyun Soon Lee ,Tel: +82-31-249-9648, Fax: +82-31-249-9139, Email: jkimtamu@kyonggi.ac.kr
Received: January 8, 2007;  Accepted: September 9, 2007.
Share :  
ABSTRACT
In an effort to identify possible microbes for seeking bioagents for remediation of herbicidecontaminated soils, seven species of phototrophic nonsulfur bacteria (Rhodobacter capsulatus and sphaeroides, Rhodospirillum rubrum, Rhodopseudomonas acidophila, blastica and viridis, Rhodomicrobium vannielii) were grown in the presence of the herbicide, butachlor, and bacterial growth rates and nitrogen fixation were measured with different carbon sources. Under general conditions, all species showed 17-53% reductions in growth rate following butachlor treatment. Under nitrogen-fixing conditions, Rb. capsulatus and Rs. rubrum showed 1-4% increases in the growth rates and 2-10% increases in nitrogen-fixing abilities, while the other 5 species showed decreases of 17-47% and 17-85%, respectively. The finding that Rp. acidophila, Rp. blastica, Rp. viridis and Rm. vannielii showed stronger inhibitions of nitrogenase activity seems to indicate that species in genera Rhodobacter and Rhodospirillum are less influenced by butachlor than those in Rhodopseudomonas and Rhodomicrobium in terms of nitrogen-fixing ability. Overall, nitrogenase activity was closely correlated with both growth rate and glutamine synthetase activity (representing nitrogen metabolism). When the carbon sources were compared, pyruvate (three carbons) was best for all species in terms of growth rate and nitrogen fixation, with malate (four carbons) showing intermediate values and ribose (five carbons) showing the lowest; these trends did not change in response to butachlor treatment. We verified that each of the 7 species had a plasmid (12.2~23.5 Kb). We found that all 7 species could use butachlor as a sole carbon source and 3 species were controlled by plasmid-born genes, but it is doubtful whether plasmid-born genes were responsible to nitrogen fixation.
Keywords: Butachlor | Herbicide | Nitrogen fixation | Plasmid | Purple nonsulfur bacteria
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
  E-Mail
Share:      
METRICS
1
Crossref
0
Scopus
1,555
View
4
Download
Editorial Office
464 Cheongpa-ro, #726, Jung-gu, Seoul 04510, Republic of Korea
TEL : +82-2-383-9697   FAX : +82-2-383-9654   E-mail : eer@kosenv.or.kr

Copyright© Korean Society of Environmental Engineers. All rights reserved.        Developed in M2community
About |  Browse Articles |  Current Issue |  For Authors and Reviewers