| Home | E-Submission | Sitemap | Contact Us |  
Environmental Engineering Research 2008;13(4): 165-168. DOI: https://doi.org/10.4491/eer.2008.13.4.165
Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances
Chris Freeman1, Inyoung Jang2, Kyoung duk Zho3, and Hojeong Kang2
1School of Biological Sciences, University of Wales, Bangor, UK
2School of Civil and Environmental Engineering, Yonsei University, Korea
3School of Public Health, Seoul National University, Korea
Corresponding Author: Hojeong Kang ,Tel: +82-2-2123-5803, Fax: +82-2-364-5300, Email: hj_kang@yonsei.ac.kr
Received: December 1, 2008;  Accepted: December 15, 2008.
Share :  
ABSTRACT
Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.
Keywords: Methylumbelliferyl phosphate | HPLC | Quench correction | Organic soil
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
  E-Mail
Share:      
METRICS
1
Crossref
0
Scopus
961
View
5
Download
Editorial Office
464 Cheongpa-ro, #726, Jung-gu, Seoul 04510, Republic of Korea
TEL : +82-2-383-9697   FAX : +82-2-383-9654   E-mail : eer@kosenv.or.kr

Copyright© Korean Society of Environmental Engineers. All rights reserved.        Developed in M2community
About |  Browse Articles |  Current Issue |  For Authors and Reviewers