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Environmental Engineering Research 2020;25(1): 62-70. DOI: https://doi.org/10.4491/eer.2018.416
A kinetic study of 4-chlorophenol biodegradation by the novel isolated Bacillus subtilis in batch shake flask
Sudhansu Sandhibigraha1, Sagnik Chakraborty2, Tarunkanti Bandyopadhyay1, and Biswanath Bhunia3 
1Department of Chemical Engineering, National Institute of Technology, Agartala, India
2School of Energy and Environmental Engineering, Hebei University of Technology, Tianjin, China
3Department of Bio Engineering, National Institute of Technology, Agartala, India
Corresponding Author: Biswanath Bhunia ,Tel: +381-2346-360, Fax: +381-2346-360, Email: bbhunia@gmail.com
Received: November 28, 2018;  Accepted: February 3, 2019.
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Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolated bacterial strain was phylogenetically related with B. subtilis. This strain was capable of breaking down both phenol and 4-CP at the concentration of 1,000 mg/L. Also, the isolated strain can able to metabolize five diverse aromatic molecules such as 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 4-nitrophenol, and pentachlorophenol for their growth. An extensive investigation was performed to portray the kinetics of cell growth along with 4-CP degradation in the batch study utilizing 4-CP as substrate. Various unstructured models were applied to evaluate the intrinsic kinetic factors. Levenspiel’s model demonstrates a comparatively enhanced R2 value (0.997) amongst every analyzed model. The data of specific growth rate (μ), saturation constant (KS), and YX/S were 0.11 h-1, 39.88 mg/L, along with 0.53 g/g, correspondingly. The isolated strain degrades 1,000 mg/L of 4-CP within 40 h. Therefore, B. subtilis MF447840.1 was considered a potential candidate for 4-CP degradation.
Keywords: Bacillus subtilis | Degradation | Genomic identification | Kinetic study | 4-chlorophenol
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