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Environmental Engineering Research 2005;10(4): 191-198. DOI: https://doi.org/10.4491/eer.2005.10.4.191
MATHEMATICAL MODEL OF SULFUR UTILIZING AUTOTROPHIC DENITRIFICATION IN AN UP-FLOW PACKED-BED REACTOR BASED ON BIOMASS DISTRIBUTION
Wooshin Park1, Yoeng-Hee Ahn2, Kyung-Ja Jung1, Rameshwar Tatavarty1, and In S. Kim1
1Bio-Environmental Engineering Laboratory, Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju, 500-712, Republic of Korea
2Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea
Corresponding Author: In S. Kim ,Tel: +82-62-970-2436, Fax: +82-62-970-2434 , Email: iskim@gist.ac.kr
Received: January 22, 2005;  Accepted: August 22, 2005.
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ABSTRACT
A novel technology for the removal of nitrogen from wastewater, an autotrophic denitrification process with sulfur particles, has been developed. A respirometer was employed to monitor the nitrogen gas produced in the reactor, while 4',6-diamidino-2-phenylindole staining was employed to investigate the biomass distribution in terms of cell number according to the reactor height. From the respirometric monitoring, the denitrification reaction was defined as a first order reaction. The reactor was divided into 7 sections and biomass was analyzed in each section where cell number was rang ed from 4.8 x 10° to 8.7 X 107 cells/g dry weight of sulfur. Cells placed mostly in the lower layer (< 10 cm of height). A function for biomass distribution was obtained with non-linear regression. Then a mathematical model has been developed by combining a plug-flow model with the biomass distribution function. The model could make a vertical profile of the up-flow packed-bed reactor resulting in a reasonable comparison with measured nitrate concentration with 5% of error range.
Keywords: Sulfur | denitrification | packed-bed | up-flow | biomass distribution | mathematical model
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